WebCycloheximide chasing assay and Western analysis were performed as in (A). The shorter and longer exposures were both shown. V, vector control. (C) An amino acid sequence alignment between the last third of PB1-F2 (HK156) and PB1-F2 (PR8) was shown in left panel. Regions of PB1-F2 (PR8) and PB1-F2 (HK156) contained in chimeric PB1-F2, as … WebAug 14, 2024 · F, (i) Cycloheximide chasing assay was performed in IM-0223. Cycloheximide (Chx, 50 μg/mL) was added after transfection of cDNA coding for human p105 and incubated for the indicated time before protein extraction. WB was performed using anti-KPC1 Ab, anti-Flag Ab (to detect exogenous p105 and p50), or anti–β-actin Ab.
Lysine 222 in PPAR γ1 functions as the key site of MuRF2 …
WebCycloheximide is a protein synthesis inhibitor in eukaryotes. Although its precise mechanism of action has yet to be fully elucidated, it has been shown to inhibit translation elongation through binding to the E-site of the … WebJul 21, 2024 · The CHX chase assay was conducted for 12 hours with following ranges : 0 hour - 1 hour - 2 hours - 4 hours - 8 hours and 12 hours. I tested a control protein (wild … table and booth
Lysine 222 in PPAR γ1 functions as the key site of MuRF2 …
WebApr 7, 2024 · A soft agar colony-formation assay was performed as follows: 1.5 ml phosphate-buffered saline (PBS; Life Technologies) containing 0.7% low melting point agarose (Lonza, Allendale, NJ) was poured into 6-well dishes (Thermo Fisher Scientific), and the layer was covered with 5.0 × 10 3 cells suspended in 3 ml medium containing … WebTo measure protein half-lives in cultured mammalian cells (HEK293), we used cycloheximide at a concentration of 100 mkg/ml. The control protein which is GFP … WebIn the present study, we demonstrate that lysine site 222 is the receptor of MuRF2-mediated PPAR γ1 ubiquitination modification, using prediction of computational models, immunoprecipitation,... table and chair cartoon