WebJul 21, 2024 · The ubiquitin-associated protein p62, which binds to LC3, is also used to monitor autophagic flux. Cells undergoing autophagy should demonstrate the co-localization of p62, LC3, and lysosomal markers. Immunofluorescence microscopy has been used to visually identify LC3 puncta, p62, and/or lysosomes on a per-cell basis.
オートファジーシグナル伝達 Cell Signaling Technology
WebMay 14, 2012 · p62はユビキチン-プロテアソームシステム (UPS) とオートファジー間のカギとなるリンクであり、ユビキチン化タンパク質のオートファジー分解、細胞内のミ … WebTo enable autophagy studies, we offer specific tools to report LC3B and p62 localization. Labeling of p62 serves as a useful marker for the induction of autophagy, clearance of … crystal springs customer service phone number
Monitoring autophagic degradation of p62/SQSTM1
WebThis system plays a crucial role in various physiological and pathological processes in living organisms ranging from yeast to mammals. Thus, an accurate and reliable measure of … WebAbstract Microtubule-associated protein light chain 3 (LC3) is now widely used to monitor autophagy. One approach is to detect LC3 conversion (LC3-I to LC3-II) by immunoblot analysis because the amount of LC3-II is clearly correlated with the number of autophagosomes. WebECU information for Ford PCM YL3F-12A650-KC. This PCM is used in Ford F-series 2000, 5.4L engine. Control unit part number. YL3F-12A650-KC. crystal springs cross country course belmont